filter sparger failures

Bioreactors Redefine Upstream Productivity

At Millipore-Sigma, says Darren Verlenden, the company's head of bioprocessing, one area of focus in bioreactor development is in new sparger and impeller designs. Their goal is to improve the volumetric mass transfer coefficient, KLa (a measure of the rate of oxygen used for fermentation) and mixing so that the bioreactors will be better able to handle increased cell densities. Get price

Fluidized bed

The resulting phenomenon is called fluidization.Fluidised beds are used for several purposes, such as fluidized bed reactors (types of chemical reactors), solids separation, fluid catalytic cracking, fluidized bed combustion, heat or mass transfer or interface modification, such as Get price

steam sparger calculations

Steam Sparger Calculation Jul 09, 2010 thread: steam flow calculation through a fixed orifice Just joined because I too am trying to double check flow calc of steam thru an orifice. Mr Rosse gave the flow in lb/hr answers for various drops but I've been struggling with Get price

Sustainable reduction of bioreactor contamination in an

2006/10/1Examples included difficulty obtaining airflow through the sparger filter post-sterilization, foaming during heat up and/or sterilization hold periods, and Templstiking failures. Documentation not only aided in contamination investigation, but also identified process-specific problems. Get price

Team:Sheffield/modelling

A ring sparger is situated at the base of the tank to provide sufficient oxygen diffusion to the tank. Aside from the tank itself a flow system including a holding tank and a recycle system with tangential flow filtration would allow for the product stream to be more effectively controlled in terms of enzyme concentration and pH (due to buffer inclusion). Get price

Bioreactor Overview

• Heating - dry heat : 180 C for 1 hr moist heat : 121 C for 30 min • Radiation kills bacteria as well as virus - X-ray,UV ray • Chemicals- formaldehyde,H2O2, ethylene oxide • Filtration- syringe filter, depth filter, screen filter 21. Contaminations in 22. Get price

Dual sparging laboratory

Perforated membrane sparger inserted in base plate of dual sparging photobioreactor: i, membrane sparger; m, perforated mem- brane; k, oriﬁce sparger. B. Six blade teﬂon impeller inserted in base plate in stirred bioreactor conﬁguration: k, oriﬁce sparger; o, impeller; s, sealed stirrer gland assembly. autoclaving 3 times in detergent (5% Deconex) and rinsed in distilled water. Get price

Gas Sparger Design

Find Gas Sparger Design related suppliers, manufacturers, products and specifications on GlobalSpec - a trusted source of Gas Sparger Design information. Celeroton AG Gas Bearing Design Challenges for Non-Air App. Gas bearings are not only applicable for air, but also for noble or inert gases.. Get price

Rules to Follow to Avoid Pump Problems

2014/8/5Most pump problems are due to suction issues. In all the years I have been in the pump business I have only found one instance of a pump problem that was related to the discharge, other than of course pumps that have had a discharge valve shut while the pump Get price

Steam Out Condition

Steam Out Condition - posted in Refining, Hydrocarbons, Oil, and Gas: hi, i have a trouble in understanding steam out condition. my current comprehension is this, as the LP steam (3.5kg/cm2 at 175C) enters the vessel will be come saturated (3.5kg/cm2 at 147C) since the vessel is vented to atmosphere during steam out. at the end of steam out, steam will be expected to be 0.5kg/cm2 at Get price

Alio Gold Inc. 2017 Foreign Issuer Report 6

2017/5/30REPORT OF FOREIGN PRIVATE ISSUER PURSUANT TO RULE 13a-16 OR 15d-16 OF THE SECURITIES EXCHANGE ACT OF 1934 For the month of May, 2017(Translation of registrant's name into English) 700 West Pender Street, Suite 615, Vancouver, British Get price

Team:Sheffield/modelling

A ring sparger is situated at the base of the tank to provide sufficient oxygen diffusion to the tank. Aside from the tank itself a flow system including a holding tank and a recycle system with tangential flow filtration would allow for the product stream to be more effectively controlled in terms of enzyme concentration and pH (due to buffer inclusion). Get price

Iron contamination / buildup problem in acid zinc plating

2008/5/1060,000 QA topics -- Education, Aloha, Fun topic 4169 Iron contamination / buildup problem in acid zinc plating A discussion started in 1999 but continuing through 2020 1999 Q. We are plating automobile components by acid zinc process. Iron inside the Get price

electro Plating: 12 Ways to Reduce Water Consumption

2009/3/12We have seen too many process failures—with associated rework, recleaning and excessive water usage—where the culprit is use of city water. Avoiding city water is particularly important in areas of poor water quality, in locations where water quality varies with the season, and in locations where requirements to manage the waste stream are, shall we say, spotty.8. Get price

GE SIEVERS INNOVOX OPERATION AND MAINTENANCE

Page 206: Gas Liquid Separator, Ndir Filter, Ndir Sample Path And Sparger Leak Check PV1-MS refers to the pneumatic valve in position 1, PV2-MS in position 2, etc. When the button is pressed, you should hear compressed air exit the selected valve, which is mounted on the left side of the Analyzer. Get price

Steam Condensation Induced Water Hammer by Wayne

Steam accident at Fort Wainwright Alaska is basis for HPAC article on steam condensation induced waterhammer by forensic engineer, Wayne Kirsner, P.E Figure 5. Steam Encroachment As Condensate is Drained Photo 1:The model pictured above was Get price

Screening proteins for NMR suitability

Filter sterilize through a 0.2 micron syringe filter into the autoclaved 15 N-labelled solution. Purification buffers Lysis Buffer: 10 mM imidazole, 20 mM tris, 500 mM NaCl, 10 uM ZnSO 4, pH 8.5 Wash Buffer : 30 mM imidazole, 20 mM tris, 500 mM NaCl, 10 uM Get price

Boiler Handbook Guide

Boiler Handbook Guide - Free ebook download as PDF File (.pdf), Text File (.txt) or read book online for free. BOILER TROUBLE SHOOTING GUIDE First edition : September 2009 By K.K.Parthiban, Boiler Specialist, Venus Energy Audit System 30/2 S.V.V Naidu Street, Pollachi -642001 India Email- parthi2006gmail / venus.energygmail Website: About the author Get price

Avoiding Pitfalls during Technology Transfer of Cell Culture

Introduction Since the early 1980s, biotechnology products have shaped the pharmaceutical industry. A large number of monoclonal antibodies and therapeutic proteins have been approved and are predicted to be the major source of revenue in upcoming years for the industry [1-5].. Get price

Practical Fermentation Technology

You can write a book review and share your experiences. Other readers will always be interested in your opinion of the books you've read. Whether you've loved the book or not, if you give your honest and detailed thoughts then people will find new books that are Get price

Practical Fermentation Technology

You can write a book review and share your experiences. Other readers will always be interested in your opinion of the books you've read. Whether you've loved the book or not, if you give your honest and detailed thoughts then people will find new books that are Get price

Fluidized bed

The resulting phenomenon is called fluidization.Fluidised beds are used for several purposes, such as fluidized bed reactors (types of chemical reactors), solids separation, fluid catalytic cracking, fluidized bed combustion, heat or mass transfer or interface modification, such as Get price

Final Report Summary

The purpose the sparger is to secure that the gas bubbles leaving the gas pipe are small to speed up the dissolution process. Unfortunately it also acts as a filter at the end of the gas pipe. To prevent clogging the average diameter of the holes in the sparger was increased from 0.01 mm to 1 mm. Get price

Generation of Infectious Feline Immunodeficiency Virus

Comparisons of the 3 dimensional structures of the two PRs led to the rational design of TL-3, a broad-based PR inhibitor capable of blocking infection by FIV, simian immunodeficiency virus (SIV), and HIV-1, as well as many drug-resistant HIV-1 variants (10, 12, 21, 23, 31, 32).). Get price

NRC: Request for Information on The Status of Licensee

3 equipment failures are described in Information Notice (IN) 87-28 and IN 87-28, Supplement 1. The staff's generic Safety Evaluation Report, NUREG-1275, V.2, was provided to all licensees and applicants by IN 87-28, Supplement 1. Get price

Deaerator

A deaerator is a device that removes oxygen and other dissolved gases from liquids. Deaerators are commonly used to remove dissolved gases in feedwater for steam-generating boilers.Dissolved oxygen in feedwater will cause serious corrosion damage in a boiler by attaching to the walls of metal piping and other equipment and forming oxides (like rust). Get price