cellulase free xylanase by thermomyces

DESIGNATION Assistant Professor EMAIL ID RESEARCH INTERESTS

Title of Ph.D. thesis : "Thermozyme: Purification and characterization of high level cellulase-free xylanase from the thermophilic fungi with special reference to Thermomyces lanuginosus" EXPERIENCE (in chronological order) Designation Type of post held Get price

Production and properties of the cellulase

Thermomyces lanuginosus IOC-4145 was able to produce a very high level of cellulase-free xylanase in shaken cultures using corncob as substrate (500 U/mL). An optimization of the medium composition in submerged fermentation was carried out aiming at a low cost Get price

Optimization, Isolation and Characterization of

Optimization, Isolation and Characterization of Cellulase–Free Thermostable Xylanase from Paenibacillus sp. Md. Abdul High Siddiqui 1, Mrityunjoy Biswas 1, Md. Omar Faruk 1, Manoranjan Roy 1, A. K. M. Asaduzzaman 1, Subed Chandra Dev Sharma 1, Topodeb Biswas 2, Narayan Roy 1 Get price

Optimization of xylanase production by Thermomyces

Thermomyces lanuginosus, a thermophilic fungus is known to produce high levels of cellulase-free xylanase in submerged culture using corncobs as carbon source (Gomes et al. 1993; Purkarthofer et al. 1993; Puchart et al. 1999; Singh et al. 2000). Get price

Optimization of upstream and downstream process

Cellulase-lacking or cellulase-poor xylanase with high temperature and pH stability has gained special attention, especially in paper and pulp industries. Most of the available literature highlighted the fungal xylanase production by optimizing environmental and cultural parameters. Get price

The growth of Thermomyces lanuginosus (TSIKL.) isolates

The lipolytic activity of Thermomyces lanuginosus strains isolated from different natural sources, International Biodeterioration Biodegradation, 55, 149-152 (2005). [15] Jatinder K., Chadha B.S., Saini H.S.: Optimization of culture conditions for production of cellulasesand xylanases by Scytalidium thermophilum using response surface methodology, World Journal of Microbiology and Get price

Production of novel alkali

Full Article PRODUCTION OF NOVEL ALKALI-THERMO-TOLERANT CELLULASE-POOR XYLANASES FROM COPRINOPSIS CINEREA HK-1 NFCCI-2032 Harjeet Kaur, * Dharm Dutt, and C. H. Tyagi Culture conditions of a newly isolated basidiomycetous strain were optimized for the enhanced production of extracellular alkali-thermo-tolerant cellulase-poor xylanase using wheat bran as the Get price

Biobleaching effects of crude xylanase from Streptomyces

Analysis of its enzymatic properties revealed that the crude xylanase possessed good thermal stability at temperatures below 60 C, exhibited a wide pH range from 4.0 to 9.0, and was cellulase-free. This crude enzyme was used to treat eucalyptus kraft pulp, and the release of chromophores was the highest at the dosage of 20 IU g -1 dry pulp. Get price

Production of a high level of cellulase

1993/8/1Thermomyces lanuginosus, isolated from self-heated jute stacks in Bangladesh, was able to produce a very high level of cellulase-free xylanase in shake cultures using inexpensive lignocellulosic biomass. Of the nine lignocellulosic substrates tested, corn cobs were found to be the best inducer of xylanase activity. The laboratory results of xylanase production have been successfully scaled up Get price

Optimization of upstream and downstream process

Cellulase-lacking or cellulase-poor xylanase with high temperature and pH stability has gained special attention, especially in paper and pulp industries. Most of the available literature highlighted the fungal xylanase production by optimizing environmental and cultural parameters. Get price

Optimization of aeration and agitation rates to improve

2003/1/1Thermostable cellulase-free xylanase was produced by Streptomyces sp. Ab106 using agricultural waste, sugar cane bagasse, as the substrate at 50 C and pH 7.0. The central composite faccentered experimental design was applied to evaluate the optimal agitation and aeration rates in a 5-l Get price

Purification and characterization of xylanases from

Xylanases produced from a locally isolated strain of Thermomyces lanuginosus and its mutant derivative were purified to a yield of 39.1 and 42.83% with specific activities of 15,501 and 17,778 IU mg−1 protein, respectively. The purification consisted of two steps i.e., ammonium sulphate precipitation, and gel filtration chromatography. The mutant enzyme showed high affinity for substrate Get price

Expression of endo

2009/6/16The produced xylanase was practically free of cellulolytic activity. The P. pastoris expression system allows a high level expression of xylanases. Xylanase was the main protein species in the culture supernatant, and the functional tests indicated that even the non-purified enzyme shows highly specific xylanase activity that is free of cellulolytic side acitivities. Get price

Optimization of xylanase production by Thermomyces

Thermomyces lanuginosus, a thermophilic fungus is known to produce high levels of cellulase-free xylanase in submerged culture using corncobs as carbon source (Gomes et al. 1993; Purkarthofer et al. 1993; Puchart et al. 1999; Singh et al. 2000). Get price

Purification and characterization of a cellulase

Thermostable cellulase-free xylanase of B. licheniformis (APX-II) from the Aureobasidium pullulans Y-2311-1. Applied and Environmental Microbiology 59, 3212–3218. Miller, G.L. 1959 Use of dinitrosalicylic acid reagent for determination of reducing sugar. Get price

Optimization of medium composition for cellulase

Economical and higher production of cellulase-free xylanase(s) is needed for their wider application in paper and pulp industries. For enzyme production at industrial scale, the growth medium contributes up to 40% of the total production cost. The Get price

Cellulase

1994/9/15Several carbohydrates were examined for their ability to induce xylanase in Thermomyces lanuginosus RT9 at 50 C in shake flask cultures. It was found that xylan or xylan containing substrates, such as corn cobs, induced maximum and comparable level of xylanase free of cellulase, while the easily metabolisable sugars (glucose, xylose, and sucrose) appeared to repress xylanase synthesis. Get price

Potential of xylanase from thermophilic Bacillus sp. XTR

Characterization of a cellulase free neutral xylanase from Thermomyces lanuginosus CBS 288.54 and its biobleaching effect on wheat straw pulp. Bioresource Technology 96, 1370–1379. Lucena-Neto, S.A., Ferreira-Filho, E.X., 2004. Get price

Production of a xylose

Cellulase levels were negligible. Biochemical characterization of H. brevis beta-glucosidase and xylanase activities showed that they were stable in a wide pH range. Optimum pH for beta-glucosidase and xylanase activities were 5.0 and 5.5, respectively, but the xylanase showed 80 % of maximal activity when assayed at pH 8.0. Get price

Improving enzymatic digestibility of wheat straw

The results indicate that the cellulase-free xylanase-secreting P. boreopolis G22 may be a potential strain for wheat straw pretreatments. The strain G22 does not only enhance the enzymatic digestibility of wheat straw, but also simultaneously produces a number of bioflocculants that can be used for various industrial applications. Get price

Thermomyces lanuginosus

Thermomyces lanuginosus is a species of thermophilic fungus that belongs to Thermomyces, a genus of hemicellulose degraders. It is classified as a deuteromycete[1] and no sexual form has ever been observed. It is the dominant fungus of compost heaps, due to its ability to withstand high temperatures and use complex carbon sources for energy Get price

Hyper production of cellulase

2009/1/1Abstract A cellulase-free xylanase production by Thermomyces lanuginosus SSBP using bagasse pulp was examined under submerged (SmC) and solid-state cultivation (SSC). Higher level of xylanase activity (19,320 37 U g −1 dried carbon source) was obtained in SSC cultures than in SmC (1,772 15 U g −1 dried carbon source) after 120 h with 10% inoculum. Get price

The growth of Thermomyces lanuginosus (TSIKL.) isolates

The lipolytic activity of Thermomyces lanuginosus strains isolated from different natural sources, International Biodeterioration Biodegradation, 55, 149-152 (2005). [15] Jatinder K., Chadha B.S., Saini H.S.: Optimization of culture conditions for production of cellulasesand xylanases by Scytalidium thermophilum using response surface methodology, World Journal of Microbiology and Get price

Bioprocess and biotechnology: effect of xylanase from

2013/8/13Yeasmin S, Kim CH, Park HJ, Sheikh MI, Lee JY, Kim JW, Back KK, Kim SH: Cell surface display of cellulase activity-free xylanase enzyme on Saccharomyces cerevisiae EBY100. Appl Biochem Biotechnol 2011, 164(3):294-304. 10.1007/s12010-010-9135-5 Get price

Cellulase

1995/9/1Thermomyces lanuginosus strains RT9 and MH4 were studied to find favourable cultivation conditions and to compare their abilities to produce xylanolytic enzymes in three media on different substrates at 50 C or 55 C under shake-culture conditions. Both organisms produced xylanases free of cellulase at widely different levels in all cultivation conditions employed. Wheat bran, corn cobs and Get price